small RNA-seq
small RNA-seq
EPIBIOTEK® small RNA-seq (miRNA regulatory network analysis included for ≥10 samples) uses QIAseq digital NGS library preparation to deliver robust quantification from low-input samples. The standard bioinformatics deliverables cover major small RNA classes and include miRNA regulatory network analysis at no additional cost.
Sample Requirements
Human, mouse, and rat; other species require evaluation
≥ 2×10^5
Cells
≥ 0.5 mg
Tissue
≥ 1 μg，RIN ≥ 6
RNA
Data Analysis
Demo
Differentially expressed small RNA heatmap
Intersection Venn diagram of small RNA target genes predicted by different software.
KEGG analysis of target genes.
GO bubble plot for target genes.
EPIBIOTEK® Project Publications
1. Bai X, Chen X, Liu X, et al. Human milk exosomal miR-148a-3p targets Casp9 to activate HIF-1 mediated apoptosis and regulate gut-lung axis hypoxic injury: A new insight into innovative infant formula improvement. Chemical Engineering Journal, 2025: 169283.
2. Wang X, Xiang Z, Zhang Y, et al. CD25 downregulation by tumor exosomal microRNA-15a promotes interleukin-17-producing γδ-T-cells-mediated radioresistance in nasopharyngeal carcinoma. MedComm (2020). 2025;6(2):e70078. Published 2025 Feb 2.
3. Chen X, Wang X, Cui Z, et al. M1 Microglia-derived Exosomes Promote Activation of Resting Microglia and Amplifies Proangiogenic Effects through Irf1/miR-155-5p/Socs1 Axis in the Retina. Int J Biol Sci. 2023;19(6):1791-1812. Published 2023 Mar 21.
4. Xu W, Yu M, Wu Y, et al. Plasma-Derived Exosomal SncRNA as a Promising Diagnostic Biomarker for Early Detection of HBV-Related Acute-on-Chronic Liver Failure. Front Cell Infect Microbiol. 2022;12:923300. Published 2022 Jul 7.