long RNA-seq
Long RNA-seq
Long RNA-seq profiles long RNAs (>200 nt) from cells or tissues, capturing both linear transcripts (mRNAs and lncRNAs) and circRNAs. It provides an efficient, quantitative overview of transcriptome-wide expression and is well suited for non-coding RNA studies. Because mRNA, lncRNA, and circRNA are obtained from the same library, it reduces sample input while improving data completeness and cost efficiency. The workflow also enables differential expression analyses across biological conditions and supports downstream target/host-gene–based functional interpretation.
Sample Requirements
Human, mouse, and rat; other species require evaluation
≥ 2×10^5
Cells
≥ 0.5 mg
Tissue
≥ 1 μg/sample; RIN ≥ 6
Total RNA
Data Analysis
Basic analysis:
1. Adapter trimming and QC of raw reads
2. Reference genome alignment
3. Expression quantification
4. Differential expression (mRNA and lncRNA)
5. GO/KEGG of differentially expressed mRNAs
6. circRNA detection/annotation
7. Differential analysis (circRNA)
8. GO/KEGG of host genes of differentially expressed circRNAs
Advanced analysis:
GSEA analysis
EPIBIOTEK® Project Publications
1. Zhang X, Zheng Y, Yang J, et al. Abnormal ac4C modification in metabolic dysfunction associated steatotic liver cells. Sci Rep. 2025;15(1):1013. Published 2025 Jan 6.
2. Teng Y, Xie R, Xu J, et al. Tubulointerstitial nephritis antigen-like 1 is a novel matricellular protein that promotes gastric bacterial colonization and gastritis in the setting of Helicobacter pylori infection. Cell Mol Immunol. 2023;20(8):924-940.
3. Zheng C, Liu M, Ge Y, Qian Y, Fan H. HBx increases chromatin accessibility and ETV4 expression to regulate dishevelled-2 and promote HCC progression. Cell Death Dis. 2022;13(2):116. Published 2022 Feb 4.