RIC-seq

RNA in situ conformation sequencing (RIC-seq) is a novel technology for capturing the higher-order structures of RNA and identifying molecular interaction sites at the cellular level. RIC-seq can investigate the conformation and organizational rules of mRNA and non-coding RNA in cells, map enhancer-promoter regulatory networks, and elucidate novel mechanisms of enhancer-mediated oncogene activation. Additionally, RIC-seq can also be used to systematically analyze the effects of disease-associated mutations on RNA higher-order structures and target interactions, which may reveal the pathogenic mechanisms of mutations in non-coding regions and lay the foundation for clinical diagnosis and treatment.