DRUG-seq

Digital mRNA with pertUrbation of Genes (DRUG-seq) is a rapid transcriptomic sequencing technology designed for low-input cell and tissue samples. The method utilizes oligo-dT magnetic beads carrying Barcode tags and Unique Molecular Identifiers (UMIs) to efficiently bind to the Poly(A) tail of mRNA for reverse transcription and library construction. The barcoded tags on the magnetic beads allow for the pooling of multiple samples into a single library, while the UMI on each sequence enables precise quantification of captured mRNA molecules, minimizing data bias caused by PCR amplification. DRUG-seq significantly reduces sample input requirements and sequencing costs, making it widely applicable in basic life science research, translational medicine, and new drug development.