ONT Direct RNA sequencing

Eukaryotic genes typically generate multiple RNA isoforms, leading to the production of functionally distinct protein variants. Traditional NGS relies on short-read RNA-seq, which often fails to capture complete transcripts or adequately reflect isoform diversity. EPIBIOTEK® introduces its ONT Direct RNA Full-Length Sequencing Service, utilizing the Oxford Nanopore Technologies (ONT) platform. This advanced service sequences RNA strands directly, leveraging long-read technology to improve isoform detection while simultaneously identifying nucleic acid modifications such as m6A, m5C, pseudouridine (Ψ), and inosine (I). This approach offers unique advantages for alternative splicing research. A single sequencing run fulfills multiple epigenomic and transcriptomic research requirements, significantly enhancing the depth, accuracy, and efficiency of the study—making it particularly suitable for multi-omics mechanisms and the analysis of precious clinical samples.