m5C BS-seq

5-Methylcytosine (m5C) is an RNA methylation modification with relatively low abundance. Nevertheless, research on its role in cancer has recently garnered substantial attention. With the emergence of high-throughput sequencing technologies, various nucleotide modifications, including m5C, can now be precisely profiled throughout the transcriptome. m5C exerts influence on RNA stability, alternative splicing, translation efficiency, and post-transcriptional regulation, thereby affecting gene expression and cellular function. Although there is extensive data regarding m5C in tRNA and rRNA, its function in mRNA remains poorly characterized, primarily due to the low abundance of m5C sites in mRNA and the absence of efficient purification strategies (1). To advance research on mRNA m5C modification, EPIBIOTEK® has recently introduced a single-base resolution mRNA m5C bisulfite sequencing technology service. m5C RNA bisulfite sequencing (m5C-BS-seq) is regarded as the gold standard for m5C mapping, as bisulfite reagents are cost-effective and non-hazardous. Moreover, PCR amplification and sequencing following bisulfite treatment ensure high sensitivity without the need for complex procedures.

1. Cells, ≥ 1.5×10⁷ cells/sample 2. Tissues, ≥ 25 mg/sample 3. Total RNA, 50 μg/sample