CUT&RUN

Cleavage Under Targets and Release Using Nuclease (CUT&RUN) is an innovative technique for detecting protein-DNA complexes within their native chromatin environment. Unlike traditional ChIP-seq, this method eliminates the need for formaldehyde crosslinking and chromatin immunoprecipitation. Consequently, CUT&RUN offers a rapid workflow, requires significantly lower cell input, and delivers superior data with high signal-to-noise ratios and excellent reproducibility. It is also highly effective for single-cell level studies. CUT&RUN represents a revolutionary advancement for research in epigenetics and gene regulation.

The CUT&RUN protocol bypasses the crosslinking and immunoprecipitation steps required by ChIP-seq. It utilizes a specific antibody against the target protein (e.g., transcription factors or chromatin remodeling proteins) to recruit a Protein A/G-Micrococcal Nuclease (pAG-MNase) fusion protein. The pAG-MNase specifically binds to the antibody and cleaves the DNA flanking the target protein, releasing the specific DNA-protein complex into the supernatant. This released DNA is then harvested for library preparation and high-throughput sequencing.